Keywords
genotoxicity
rambutan peel extract
How to Cite
Abstract
Acrylamide (AA) is a toxic compound that can be produced in foods containing reducing sugars and amino acids (asparagine) when cooked at high temperatures. Furthermore, rambutan peel extract (RPE) is a source of various bioactive compounds with recognized antioxidant activity. The objective of this study was to profile the antioxidant activity of RPE (4–64 μg/mL) and its LC50 in a primary leukocyte culture to ensure its safety and subsequently evaluate its genoprotective effect against the damage caused in an in vivo toxicity model, in Wistar rats exposed to AA (20 mg/kg bw). Viability greater than 75% was observed after exposure of leukocytes to ECR at all concentrations used, so it was considered non-toxic. An LC50 of 94.61 μM was established for AA in this experimental model. Furthermore, ECR provoked a notable decrease in DNA damage was observed, evidenced by the decrease in micronucleus (MN) formation in polychromatic erythrocytes (PE) from the bone marrow of Wistar rats subjected to oral exposure to AA. Based on the above, it was concluded that the extract is non-toxic at the concentrations used and also exhibits a notable genoprotective effect against AA damage, which highlights it as a potential nutraceutical.
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